Your privacy, your choice

We use essential cookies to make sure the site can function. We also use optional cookies for advertising, personalisation of content, usage analysis, and social media.

By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some third parties are outside of the European Economic Area, with varying standards of data protection.

See our privacy policy for more information on the use of your personal data.

for further information and to change your choices.

You are viewing the site in preview mode

Skip to main content
Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Plasma-derived extracellular matrix for xenofree and cost-effective organoid modeling for hepatocellular carcinoma

Fig. 2

A. Phase-contrast micrograph of the HUH-7, HUVEC, and BM-MSCs cells (a-c). B. Micrograph of 3D HUH-7 organoids and C. 3D Mixed organoids, (a) stained with Calcine AM (b) Hoechst (c) and merged (d) H & E stained cryosections show the structure and lumen (*) of the 3D Mixed organoids surrounded by a network of stromal cells (e). D. Flow cytometry analysis of cell viability using propidium iodide staining. (a) HUH-7 organoids (b) 3D Mixed organoids after 14 days. E. RT-qPCR shows relative levels of expression of cell cycle regulator (CCNA1, CCNB1, CCND, and CCNE), proliferation (BAX, BCL2), and apoptosis genes (P21, P53, APAF1) in HUH-7 2D, 3D HUH-7, 3D Mixed, and HCC tissue. Values represent relative gene expression mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001 respectively

Back to article page

Journal of Translational Medicine

ISSN: 1479-5876

Contact us