Fig. 3

Invasion potential. (A) Gene expression of EMT markers and invasion markers in HUH-7 2D), organoids (3D HUH-7 and 3D Mixed organoid groups, and HCC tissue. Values represent relative gene expression mean ± SD. ^*p < 0.05, ^**p < 0.01, and ^***p < 0.001 respectively. (B) H&E stained cryosections micrograph of (a) 3D HUH-7 (b) 3D Mixed organoid groups after 14 days show protrusive organoid structure with cytoplasmic filaments (arrow) invading the biomatrix