Fig. 5

CSC markers. A, B. Flow cytometry analysis of CD44⁺/CD24⁺ cell population shows significantly higher CD44⁺ population in the 3D Mixed compared to 3D HUH-7 organoids. C. Fluorescent micrographs for the organoids generated from day 14. 3D HUH-7 and 3D Mixed organoids stained for EPCAM (a, b) and CK19 (c, d). D. Gene expression analysis of CSC markers in 2D cultured HUH-7 cells, 3D HUH-7, 3D Mixed organoids, and HCC tissue. Values represent relative gene expression mean ± SD. ^*p < 0.05, ^**p < 0.01, and ^***p < 0.001 respectively