Fig. 6

Chemotherapy resistance. A. Fluorescent micrograph of 3D HUH-7 (a) and 3D Mixed organoids (b) stained with Calcein AM after treatment with Doxorubicin (2 μm) for 72 h. B. Gene expression analysis of proliferation (BCL2, BAX), apoptosis (P53, P21), and drug resistance (ABCG2) markers in HUH-7 cells cultured in HUH-7 2D, 3D HUH-7, 3D Mixed organoid, and HCC tissue. C. MTT assay for HUH-7 cells treated with various concentrations of doxorubicin for 72 h. For figures B and C, values represent the mean of absorbance ± SD. ^*p < 0.05, ^**p < 0.01, and ^***p < 0.001 respectively. D. Identification of side population (SP) cells from 3D HUH-7 (a) and the 3D Mixed organoids (b)