Table 1 Sequence of the primers used
Name | F/R | 5` - Oligo Seq − 3` | Name | F/R | 5` - Oligo Seq − 3` |
|---|---|---|---|---|---|
CCNA1 | F | GCACACTCAAGTCAGACCTGCA | EPCAM | F | CGCAGCTCAGGAAGAATGTG |
R | ATCACATCTGTGCCAAGACTGGA | R | TGAAGTACACTGGCATTGACG | ||
CCNB1 | F | GACCTGTGTCAGGCTTTCTCTG | ABCG2 | F | TTTCCAAGCGTTCATTCAAAAA |
R | GGTATTTTGGTCTGACTGCTTGC | R | TACGACTGTGACAATGATCTGAGC | ||
CCND2 | F | GCTGTCTCTGATCCGCAAGC | CA9 | F | GTGCCTATGAGCAGTTGCTGTC |
R | AGGGCATCACAAGTGAGCGA | R | AAGTAGCGGCTGAAGTCAGAGG | ||
CCNE1 | F | TGTGTCCTGGATGTTGACTGCC | WNT7B | F | CCTCCCTGGATCATGCACAG |
R | CTCTATGTCGCACCACTGATACC | R | CACGTACAGGACGCCAAAGC | ||
APAF1 | F | GCCAAGCAGGAGGTCGATAATG | APOBEC3B | F | GCGCCAGACCTACTTGTGCT |
R | GACCATCCTCAGAAAAGCAGGC | R | CCGGGTCCAACTCGTTGCATAG | ||
B-actin | F | AGAGCTACGAGCTGCCTGAC | DLG5 | F | TCAGCAGTGTGGGCACTACC |
R | AGCACTGTGTTGGCGTACAG | R | AAAGGCCGTGCCATGCGTAG | ||
TP53 | F | GTTCCGAGAGCTGAATGAGG | APOBEC3B | F | GCGCCAGACCTACTTGTGCT |
R | TTATGGCGGGAGGTAGACTG | R | CCGGGTCCAACTCGTTGCATAG | ||
BAX | F | CAAACTGGTGCTCAAGGCCC | GABRD | F | ACCACACCAACGAGACCCTG |
R | GAGACAGGGACATCAGTCGC | R | GCAGCCGGATGAGCTTGTTC | ||
BCL2 | F | GGATAACGGAGGCTGGGATG | FAM186A | F | AACCCACGCTTTGGAGTCCC |
R | TGACTTCACTTGTGGCCCAG | R | TTGCTTAAGGGTGAGGGGCG | ||
MMP-2 | F | TTCACCCACATCAGGAACCCC | TGF-α | F | CGCCCGTAAAATGGTCCCCT |
R | ACTGCCTTCGATACACCGGG | R | GGCACGCAGCCAACACAATA | ||
MMP-3 | F | CACTCACAGACCTGACTCGGTT | IGF2 | F | TGGCATCGTTGAGGAGTGCTGT |
R | AAGCAGGATCACAGTTGGCTGG | R | ACGGGGTATCTGGGGAAGTTGT | ||
MMP-13 | F | TTCGGCTTAGAGGTGACTGGC | TNFSF10 | F | TGGCAACTCCGTCAGCTCGTTA |
R | TTCACCCACATCAGGAACCCC | R | AGCTGCTACTCTCTGAGGACCT | ||
E-cadherin | F | GTCACTGACACCAACGATAATCCT | KRAS | F | TGTTCACAAAGGTTTTGTCTCC |
R | TTTCAGTGTGGTGATTACGACGTTA | R | CCTTATAATAGTTTCCATTGCCTTG | ||
SNAIL | F | ACCACTATGCCGCGCTCTT | CD24 | F | TGCTCCTACCCACGCAGATT |
R | GGTCGTAGGGCTGCTGGAA | R | GGCCAACCCAGAGTTGGAA | ||
Vimentin | F | TGTCCAAATCGATGTGGATGTTTC | CD44 | F | AGAAGGTGTGGGCAGAAGAA |
R | TTGTACCATTCTTCTGCCTCCTG | R | AAATGCACCATTTCCTGAGA | ||
AFP | F | AGCAGCTTGTTAAATCAACATGCA | RHOA | F | GGCAAACAGGATTGGCGCTT |
R | AAAATTAACTTTGGTAAACTTCTGACTCAGT | R | CCGCATAAGGGCTGTGCTTG | ||
TGFB | F | CAGCAACAATTCCTGGCGATA | P21 | F | TGGAACTTCGACTTTGTCAC |
R | AAGGCGAAAGCCCTCAATTT | R | CACATGGTCTTCCTCTGCT | ||
TCF4 | F | GCCTCTTCACAGTAGTGCCATG | C-MYC | F | AAACACAAACTTGAACAGCTAC |
R | GCTGGTTTGGAGGAAGGATAGC | R | ATTTGAGGCAGTTTACATTATGG | ||
CD133 | F | CAGAGTACAACGCCAAACCA | |||
R | AAATCACGATGAGGGTCAGC | ||||