Fig. 5

frzb functioned during PEMFs exposure to enhance BMSCs chondrogenic differentiation and was related to Wnt-βcatenin signaling pathway. (A) Clustering heatmaps illustrated the expression levels of differential genes in day 7 and day 14 (n = 3). (B) Gene Ontology (GO) analysis of upregulated genes in pellets transcriptome (Top 20). (C) A volcano plot illustrated three targeted genes in the upregulated site (frzb, plau, sost). (D) Quantification of the transcript levels of frzb, plau and sost of day 7 pellets. (E) Quantification of the transcript levels of frzb, plau and sost of day 14 pellets. (F) For day 7 pellets, the protein levels of β-catenin and sFRP3 and quantification of the relative expression level of β-catenin and sFRP3. (G) For day 14 pellets, the protein levels of β-catenin and sFRP3 and quantification of the relative expression level of β-catenin and sFRP3. ns, p>0.05; * p<0.05; ** p<0.01; *** p<0.001. Student’s t test and one-way ANOVA were used for comparison between two groups and multiple groups, respectively