Fig. 5

miR-223 induces the upregulation of ABCA1, reduces LDs accumulation, promotes myelin debris degradation and enhances cholesterol efflux in myelin debris triggered-BV2. (A) IF staining of LDs (BODIPY, orange) and ABCA1 (green) in the afore-mentioned 4 groups. (B) Quantification analysis of the mean fluorescence intensity (MFI) of Bodipy and ABCA1 (n = 4). (C) IF staining of myelin debris (MBP, orange) and IL-6 (red) in the afore-mentioned 4 groups. (D) Quantification analysis of the MFI of MBP and IL-6 (n = 4). (E) Quantitative analysis of total cholesterol content in BV2 cells and supernatant (n = 5). (F/G) Quantitative analysis of ABCA1, IL-1β and TNF-α expression in BV2 cells using Western blot analysis normalized with the housekeeping protein β-tubulin in the six groups mentioned above (n = 4). Scale bars, 10 μm. ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001