Fig. 7

Cardiac Tissue Staining in Different Rat Groups. (A) Oil Red O staining of cardiac tissues, with the first row scale bar = 1000 μm and the second row scale bar = 100 μm. (B) Masson’s trichrome staining of cardiac tissues, first row scale bar = 1000 μm, second row scale bar = 100 μm. (C) WGA staining of cardiac tissues, scale bar = 100 μm. (D) Immunohistochemical staining for p16 in cardiac tissues, scale bar = 50 μm. (E) Immunohistochemical staining for p21 in the heart, scale bar = 50 μm. (F) Immunohistochemical scoring for p16 and p21 in cardiac tissues (n = 5). (G) Semi-quantitative analysis of Oil Red O staining in the heart (n = 5). (H) Quantification of myocardial collagen volume fraction in cardiac tissues (n = 5). (I) Cross-sectional area of cardiac myocytes (n = 25). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001