Fig. 1

Behavioral tests and TH protein detection were used to assess the successful construction of the chronic PD mouse model. A Schematic of the construction of the chronic PD mouse model. B, C Open field test to detect the locomotor activity of mice. D Grasping test to detect the coordination ability of mice. E Rotarod test to detect the locomotor activity of mice. F WB to detect TH protein expression. G Statistical graph for TH protein expression. H IHC detection TH positive dopaminergic neurons; Scale bar: 200 µm. I Statistical chart of average optical density. Behavioral tests n = 10/group. Data are presented as mean ± standard deviation. Unpaired t-test was used for comparison between two groups. ***P < 0.001, ****P < 0.0001