Fig. 3

The high expression of miR-186-3p in PD cellular models promotes the activation of apoptotic pathways, leading to increased levels of dopaminergic neuron apoptosis. A KEGG analysis of the functions enriched by the target genes possibly regulated by miR-186-3p. B Detection of miR-186-3p transfection efficiency using FISH; Scale bar: 10 µm. C Statistical graph of fluorescence intensity. D RT-qPCR to detect miR-186-3p transfection efficiency. E WB analysis was used to detect the expression of TH protein in PD cell models after transfection with miR-186-3p. F TH protein expression statistical graph. G Detection of cell apoptosis levels using FCM assay in PD cell models after transfection with miR-186-3p. H Statistical graph of apoptosis levels. I Detection of cell viability using CCK8 assay. J WB analysis for the detection of P-PI3K, PI3K, P-AKT and AKT protein expression. K, L P-PI3K and P-AKT protein expression statistical graph. M WB analysis for the detection the expression of Cleaved caspase-3, Cytochrome c, Bax, and Bcl-2 protein. N, O Protein expression statistical graph. Data are presented as mean ± standard deviation. One-way ANOVA was used to compare differences among multiple groups. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ^*P < 0.05 inhibitor group vs. NC group.. ^#P < 0.05 mimic group vs. NC group