Fig. 4

Knocking down the expression of miR-186-3p in dopaminergic neurons in the midbrain tissue of PD mice can enhance the motor ability of mice and reduce the degeneration and death of dopaminergic neurons. A Schematic of AAV stereotaxic injection in PD mice. B Open field test to detect the locomotor activity of mice. C Statistical graph of total distance. D Grasping test to detect the coordination ability of mice. E Rotarod test to detect the locomotor activity of mice. F Pole-climbing test to detect the locomotor activity of mice; and mouse behavioral tests (n = 15/group). G WB to detect the expression of TH and α-syn proteins in the midbrain tissue of mice. H, I Statistical graph for TH and α-syn protein expression. J IF detection and co-localization of AAV and TH protein. Scale bar: 10 µm. K Statistical chart of average fluorescence intensity. L RT-qPCR to detect the expression of miR-186-3p in the midbrain tissue of mice. Data are presented as mean ± standard deviation. One-way ANOVA was used to compare differences among multiple groups. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001