Fig. 5

Knocking down the expression of miR-186-3p in dopaminergic neurons of PD mouse midbrain tissue can inhibit the activation of apoptosis pathways and increase the content of dopamine and its metabolites. A WB analysis for the detection the expression of P-PI3K, PI3K, P-AKT and AKT protein. B, C P-PI3K and P-AKT protein expression statistical graph. D WB analysis for the detection the expression of Cleaved caspase-3, Cytochrome c, Bax and Bcl-2 protein. E, F Protein expression statistical graph. G Dopamine peak chart. H LC–MS/MS detection of dopamine content in midbrain tissue. I Homovanillic acid peak chart. J LC–MS/MS detection of homovanillic acid content in midbrain tissue. K Tyrosine peak chart. L LC–MS/MS detection of tyrosine content in midbrain tissue. Data are presented as mean ± standard deviation. One-way ANOVA was used to compare differences among multiple groups. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001