Fig. 6

MiR-186-3p binds to the 3’UTR regions of slc6a3 and igf1r genes, inhibiting the expression of target genes in PD cellular models. A Bioinformatics analysis of target genes regulated by miR-186-3p. B Bioinformatics analysis of target gene expression in PD model. C The Venn diagram presents the potential target genes slc6a3 and igf1r that may be regulated by miR-186-3p. D WB to detect IGF1R and DAT protein expression in the PD cell model. E IGF1R and DAT protein expression statistical graph. F WB to detect IGF1R and DAT protein expression in the PD mouse model. G IGF1R and DAT protein expression statistical graph. H Binding site of miR-186-3p with the 3’UTR region of the slc6a3 gene. I Luciferase activity of igf1r reporter after co-transfection with miR-186-3p. J Binding site of miR-186-3p with the 3’UTR region of the igf1r gene. K Luciferase activity of igf1r reporter after co-transfection with miR-186-3p. Data are presented as mean ± standard deviation. One-way ANOVA was used to compare differences among multiple groups. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001