Table 1 Zebrafish primers and target sequences for gRNA
From: Spastin accumulation and motor neuron defects caused by a novel SPAST splice site mutation
CRISPR gRNA | gRNA target sequence | qPCR forward (F) and reverse (R) primers |
|---|---|---|
Target1 | TGACGCAGTGGCAGTGGTAC TGG | F: AGAAGGTTTGTCTCACAGGGTC R: AACTGCAATATGCACCTAAACC |
Target2 | CCTCATCCTCAACGAGATCG TGG | |
Target3 | CCACGATCTCGTTGAGGATG AGG | |
Target4 | TCCTCAACGAGATCGTGGAC AGG |