Fig. 5

Fibroblast subtype transcription factor identification and regulon activity scoring. A The activity distribution of all transcription factors. B The activity distribution of the top 10 transcription factors highly expressed in each subtype. C Bubble heatmap showing the activity of specific transcription factors in each fibroblast subtype. The size of the dot indicates the degree of specificity (RSS: regulation specificity score), and the color of the dot indicates the level of activity (RAS: regulon activity score). D Scatter plot showing the specific ranking of transcription factors in each fibroblast subtype. The horizontal axis represents the ranking position, and the vertical axis represents the specificity. E Distribution diagram of AUC values of four representative transcription factors screened in the BNIP3+ Fibro subtype in all cells. The dotted line, which is the AUC value, is the threshold for determining that cells are specific for this transcription factor. F Expression distribution of transcription factors on the UMAP plot. G Expression distribution of transcription factors and target gene sets on the UMAP plot