Fig. 4
Relationship of malignant cell heterogeneity with immune microenvironment reprogramming. a t-SNE visualization of T, myeloid, and B cells, colored by cell subsets. CD8 Tex: exhausted CD8 + T cells; Prol Tex: proliferative exhausted cells; TAM: tumor-associated macrophages; DC: Dendritic cell. b Box plots showing the differences in the infiltrating proportion of CD8 Tex, Prol Tex and Treg between ITMHhi and ITMHlo tumors in single-cell cohort. c Box plots showing the differences in the exhaustion, immunosuppression, and cytotoxicity score between ITMHhi and ITMHlo tumors in the single-cell cohort. d Heatmap showing the infiltrating immune composition using CIBERSORT and ssGSEA. e Heatmap showing the relative activity of transcription factor in T cell subsets. f Scatter plot showing the outgoing and incoming interaction strength of each cell subset in ITMHlo tumors and ITMHhi tumors. g Dot plot of ligand-receptor interaction from tumor cells to immune cells between ITMHhi tumors and ITMHlo tumors; dot size represents the P-value, and color represents the interaction strength