Fig. 3

CSF influx decreases after depletion of PBM. A Schematic of experiment. B Representative images of brain sections showing CD206 and lectin staining. Scale bar = 200 μm. C Quantification of the coverage of CD206⁺ cells. n = 6 per group. D Schematic of experiment. E Representative images of fluorescent tracer distribution within 30 min after CM injection. Scale bar = 2 mm. The dashed area represents the ROIs included in the quantification. F Influx area of fluorescent tracer during the 30 min after CM injection, quantified from the ROIs in (E). n = 5 per group. G Quantification of the influx area of fluorescent tracer at 30 min. n = 5 per group. H Representative images of fluorescent tracer coverage in brain sections. Scale bar = 2 mm. I Quantification of the fluorescent tracer coverage. n = 5 per group. J Representative images of AQP4 expression in the cerebral cortex. Scale bar = 100 μm. K Quantification of AQP4 coverage. L Quantification of the polarization index of AQP4. For K and L. n = 6 per group. Data are presented as mean ± SEM, unpaired t-test. **P < 0.01, ***P < 0.001, ns = not significant