Fig. 7

Mfsd2a inhibits CRLM via the S100A14/STAT3 axis. (A) Protein expression levels of STAT3 and P-STAT3 were assessed in CRC cells co-transfected with Mfsd2a overexpression and/or S100A14 knockdown. (B) Immunofluorescence intensity of Mfsd2a, S100A14, and P-STAT3 was evaluated in CRLM models established with CT26 cells. (C) Transwell assays demonstrated that colivelin rescued the inhibitory effects of Mfsd2a overexpression on CRC cells migration and invasion. (D) Immunofluorescence analysis was performed to examine the expression of EMT-related proteins in CRC cells. (E) Western blot analysis was conducted to determine the expression levels of Mfsd2a, S100A14, and EMT-related proteins in CRC cells. (F,G) CRLM models showed that colivelin partially counteracted the inhibitory effect of Mfsd2a on liver metastasis formation. Mice were intraperitoneally injected with 1 mg/kg colivelin or DMSO every other day for 2 weeks. (*P < 0.05, **P < 0.01, ***P < 0.001,****P < 0.0001)