Fig. 1

Transcriptomic analysis of the striatal in Hemi-PD Rats following TAL treatment. A Immunostaining of TH in the striatum of Hemi-PD rats (Scale bar: 1000 μm, N = 8/group, mean ± SEM). Statistical significance: ****P < 0.0001, Student’s t-test. B Adjusting step tests of Hemi-PD Rats treated with saline or TAL (5 mg/kg, i.p.). N = 8. C, D Volcano plots of DEGs. Comparison of gene expression changes between the lesioned side of the control group (CTR-Les) and the intact side of the control group (CTR-Int), the lesioned side of the TAL-treated group (TAL-Les) and the CTR-Les group. Blue dots represent downregulated DEGs, and red dots represent upregulated DEGs (q-value < 0.05, |log2FC |≥ 2). E RT-PCR analysis shows the expression levels of trhr, rarα, creb and camk2b mRNA in the striatum (N = 8/group, mean ± SEM). Statistical significance: **P < 0.01, ****P < 0.0001, one-way ANOVA followed by Tukey's test. F Western blot analysis of CREB and CAMK2B levels in the striatum of different groups of rats (N = 8/group; error bars represent SEM). Statistical significance: *P < 0.05, **P < 0.01, ****P < 0.0001, one-way ANOVA followed by Tukey's test. I, J Gene Ontology (GO) enrichment analysis of transcripts. The X-axis represents the enrichment ratio, the Y-axis represents the GO cellular component term, and the size of the bubbles indicates the number of DEGs annotated to a particular term. The color represents the P-value of enrichment, with a smaller P-value indicated by a redder color