Fig. 2
Pegmolesatide has a stronger ability to maintain UT-7 cell proliferation than ESPO 3000 at matched biological doses. A and B Effect of drugs on the proliferation of UT-7 cells. UT-7 cells in logarithmic growth phase were adjusted to 1.2 × 104cells/mL with complete medium without EPO. Cells were treated with drugs (Pegmolesatide or ESPO3000 or peptide dimer) for 0, 1, 2, 3, 4, 5, 7, 8, and 9 days. C The effect of drugs on cell survival. After treating cells with drugs for 7 days, centrifuge and discard the supernatant, adjust the cell density to 1.0 × 105cells/mL with complete medium without EPO. Continue to culture the cells for 0, 1, 2, 3, 4, and 7 days. Use Cell Titer-Glo® 2.0 Cell Viability Assay to detect cell luminescence signals. The magnitude of the signal value is proportional to the number of viable cells. Data are presented as Mean ± SD. N = 3 per time point