Fig. 6

ASPN is an early upregulated marker in the CAG stage of gastric tumorigenesis. (A) Venn plot of conservatively upregulated markers, color-coded by patient. (B) Feature plot of ASPN gene expression (gray to red) in fibroblast clusters; CAG: left panel; control: right panel. (C) Boxplots showing ASPN expression levels between different disease conditions in public datasets (GSE130823, GSE191275, TCGA-STAD dataset). (D) Boxplot showing ASPN expression levels between different disease conditions in GSE153224. (E) Boxplot showing ASPN expression levels detected by RT‒PCR in CAG and corresponding control samples (n = 5). P values were calculated via the two-sided Wilcoxon test. (F) Volcano plot showing the DEGs between APSN-positive CCL11+APOE+ fibroblasts and ASPN-negative CCL11+APOE+ fibroblasts. (G) Representative mIHC staining of ASPN. CAG and control tissues were stained with DCN (red), ASPN (green), and DAPI for DNA (blue)