Fig. 5

CBR3-AS1 participates in the radioresistance of H520 cells by regulating the miR-409-3p and CXCL1 axis. A, B The viability and proliferation of H520 cells were assessed using the CCK8 and colony formation assays, respectively. C Invasion ability of H520 cells. D Scratch wound healing in H520 cells at 0 and 24 h. E Apoptosis levels in H520 cells as assessed via flow cytometry. H520 cells were transfected with shCBR3-AS1, shmiR-409-3p, CBR3-AS1, or miR-409-3p, and then exposed to radiation. The significance between two groups was analyzed using Student's t-test. Data are presented as mean ± SD from three independent experiments. Statistical significance was defined as *P < 0.05, **P < 0.01, and ***P < 0.001