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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: FSTL1 accelerates nucleus pulposus-derived mesenchymal stem cell apoptosis in intervertebral disc degeneration by activating TGF-β-mediated Smad2/3 phosphorylation

Fig. 3

FSTL1 expression is induced in NP tissues in the IVDD rat model. (A, B) Representative T2-weighted MRI shown for control (CTR) and annulus fibrosus punctured (AFP) rats, and quantitative comparisons of Pfirrmann grade. (C, D) Representative sections of NP tissues stained with HE, SO and Alcian blue (scale bar: 1 mm) and corresponding histological scores. (E) NP-MSCs isolated from the groups in (A) were subjected to flow cytometry to measure characteristic positive (CD105, CD90, CD73) and negative (CD34, CD45 and HLA-DR) markers of NP-MSCs. Representative histograms compare marker staining against an isotype control. (F, G) Representative immunofluorescence staining against FSTL1 in the cells from (E) (scale bar: 50 μm) and corresponding quantitative analysis. (H, I) Western blotting against FSTL1 in the cells from (E) and quantitation of relative FSTL1 protein expression. (J) RT-qPCR measurements of relative FSTL1 mRNA in the cells from (E). Values are mean ± SD for n = 3 determinations. *p < 0.05, **p < 0.01, ***p < 0.001

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Journal of Translational Medicine

ISSN: 1479-5876

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