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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: FSTL1 accelerates nucleus pulposus-derived mesenchymal stem cell apoptosis in intervertebral disc degeneration by activating TGF-β-mediated Smad2/3 phosphorylation

Fig. 5

Knockdown of FSTL1 using siRNA prevents acid-induced ECM degeneration and apoptosis in NP-MSCs. (A-D) Normal NP-MSCs exposed to control or pH 6.6 conditions or transfected with control or FSTL1 targeting siRNAs before pH 6.6 treatment were cultured for 24 h before assessing the rates of apoptosis using TUNEL assays (A, B) or flow cytometry measuring Annexin V/PI staining (C, D). Representative epifluorescence images (A) and quantitative analysis of TUNEL-staining positive cells (B) (scale bar: 100 μm), representative flow dot plots (C) and quantitation (D). (E-N) Western blotting against FSTL1 and apoptosis-related proteins (cleaved-Caspase3, Bax, and Bcl-2) (E) or ECM-related proteins (Collagen II, Aggrecan, MMP3, and MMP13) (J) with respective quantitation of protein level changes shown in (F-I) and (K-N). The values are mean ± SD for n = 3. *p < 0.05, **p < 0.01, ***p < 0.001

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Journal of Translational Medicine

ISSN: 1479-5876

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