Fig. 4

Characterization of Leukocytes-derived EVs from healthy controls biofluids. A NTA tracking of size (diameter/nm) and concentration (particles/mL) of pure total EVs population sorted from PB (left) and tears (right). EVs derived from total PB or total tear showed a median diameter of 120.3 nm and 105.4 nm, respectively, falling within the size range detected by AFM reported in the figure. The height cross sectional profiles of representative vesicles of total PB (blue arrow) and total tear (red arrow) are also reported. B NTA tracking of size (diameter/nm) and concentration (particles/mL) of pure Leuko EVs sorted from PB (left) and tears (right). Leuko EVs derived from PB or tears showed a median diameter of 127.5 nm and 145.8 nm respectively, falling within the size range detected by AFM indicated in the figure. The height cross sectional profiles of representative Leuko EVs of PB samples (green arrow) and tears (orange arrow) are also reported