FACS-Proteomics strategy toward extracellular vesicles single-phenotype characterization in biological fluids: exploring the role of leukocyte-derived EVs in multiple sclerosis

Table 4 Differential proteins in Leuko EVs isolated from tears of RRMS patients compared to HCs

Expression in RRMS	Gene names	Description	−Log(p-value)	Difference
Down	GGCT	Gamma-glutamylcyclotransferase	1.83	− 0.26
Up	LDHA	L-lactate dehydrogenase A chain	3.59	0.66
Down	LTF	Lactotransferrin	3.11	− 1.00
Up	CAT	Catalase	2.52	0.89
Up	ALDOA	Fructose-bisphosphate aldolase A	1.53	0.34
Up	ANXA1	Annexin A1	2.46	1.50
Down	S100A8	Protein S100-A8	2.81	− 0.77
Up	ANXA2	Annexin A2	4.05	0.67
Up	HSP90AB1	Heat shock protein HSP 90-beta	2.56	0.76
Up	VIM	Vimentin	4.90	1.85
Up	UBA52	Ubiquitin-60S ribosomal protein L40	2.87	0.95
Up	PKM	Pyruvate kinase PKM	2.37	0.48
Up	FLNA	Filamin-A	1.93	1.10
Down	SPRR2A	Small proline-rich protein 2A	2.11	− 0.72
Down	CALML3	Calmodulin-like protein 3	2.12	− 1.28
Down	SERPINB3	Serpin B3	2.37	− 0.58
Down	S100A7	Protein S100-A7	3.23	− 0.96
Down	SFN	14-3-3 protein sigma	1.81	− 1.06
Up	MYH9	Myosin-9	2.83	1.33
Up	DEFA3	Neutrophil defensin 3	4.42	1.21
Up	ACTG1	Actin, cytoplasmic 2	4.40	1.66
Down	HIST1H4A	Histone H4	4.16	− 1.22
Up	HBB	Hemoglobin subunit beta	4.11	0.96
Up	DCD	Dermcidin	1.52	0.42
Up	PRDX1	Peroxiredoxin-1	2.59	0.81
Down	TGM3	Protein-glutamine gamma-glutamyltransferase E	2.46	− 0.41
Up	DSC1	Desmocollin-1	2.48	0.69
Down	PKP1	Plakophilin-1	1.47	− 0.33
Down	BLMH	Bleomycin hydrolase	1.33	− 1.27
Up	SBSN	Suprabasin	2.57	0.88
Up	SERPINB12	Serpin B12	1.82	0.22
Down	LACRT	Extracellular glycoprotein lacritin	1.38	− 0.55
Down	DMBT1	Deleted in malignant brain tumors 1 protein	3.09	− 1.22

Leuko EV proteins significantly up- and down-regulated in pooled RRMS tears are indicated by red and green dots in the Volcano Plot, respectively, according to p-value and “Difference” values reported in the table

ISSN: 1479-5876